mouse anti-v5 primary antibody (Thermo Fisher)
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mouse anti-v5 primary antibody
Mouse Anti V5 Primary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-v5+primary+antibody/anti+v5/bio_rxiv__2025__07__16__665091-227-9-15
Average 90 stars, based on 1 article reviews
Mouse Anti V5 Primary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-v5+primary+antibody/anti+v5/bio_rxiv__2025__07__16__665091-227-9-15
Average 90 stars, based on 1 article reviews
mouse anti-v5 primary antibody - by Bioz Stars,
2026-09
90/100 stars
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Recombinant:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Transfection:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Expressing:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Immunodetection:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Plasmid Preparation:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Immunocytochemistry:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Functional Assay:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Luciferase:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Negative Control:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a RNA Binding Assay:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Derivative Assay:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Binding Assay:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Activity Assay:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Construct:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Labeling:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Western Blot:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Mutagenesis:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a FLAG-tag:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Positive Control:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Two Tailed Test:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Knock-Out:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Incubation:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Blocking Assay:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a In Silico:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Fluorescence:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Staining:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a Immunofluorescence:Article Title: LOC689986 , a unique gene showing specific expression in restricted areas of the rodent neocortex Article Snippet: Detection of recombinant protein was achieved either directly (transient transfection using vectors encoding either a C- or N-terminal YFP tagged recombinant protein), or by using Article Title: Subcellular proteomics of Paradiplonema papillatum reveals digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans Article Snippet: The slides were incubated with either mouse anti-V5 or rabbit anti-V5 primary antibody diluted (1:500; Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4–NOT deadenylase complex subunits to repress target mRNAs Article Snippet: Article Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. Article Snippet: Article Title: The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity Article Snippet: Mouse anti-v5 primary antibody (invitrogen) at a 1:10000 dilution, Article Title: Mutations in DCHS1 Cause Mitral Valve Prolapse Article Snippet: Western blots were probed with either a Article Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals Article Snippet: Fixed cells were rinsed three times with PBS and blocked with 3 % BSA in PBS for at least 1 h. Cells transfected with Ankle1-V5 constructs were incubated with a Article Title: Primary cilia defects causing mitral valve prolapse. Article Snippet: Western blots were probed with a |
![Insertion of <t>V5</t> tag at the N-terminus of capsid enables successful detection of the capsid. (A) Sequence alignment between a region of VEEV TC-83 and VEEV Cm showing key mutation sites [created in BioRender. Kehn-hall, K. (2026), https://BioRender.com/d8zhltb ]. (B,C) Schematic of VEEV and VEEV Cm with V5 tag inserted at the N-terminus of the capsid protein [created in BioRender. Kehn-hall, K. (2026), https://BioRender.com/nk52hc0 ]. (D,E) Graphs depicting the replication kinetics of V5-C and V5-Cm relative to their respective parental viruses. Vero cells were infected at an MOI 1, and viral titers were determined by plaque assay at 4, 6, 8, 12, and 24 hpi. ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are represented as mean ± SD. N = 3. (F–I) Western blots for V5-C and V5-Cm expression at various time points (MOI 5) in HMC3 and Vero cells. E1-V5 sample was used as a positive control. Mock-infected cells were used as a negative control, and actin was probed as a loading control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7339/pmc13057339/pmc13057339__fmicb-17-1784611-g001.jpg)
